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Figure 5B suggests representative spectra out of nitroxide (CM.) signals out of PBS- and you will Bleo-treated rats, respectively. Mitochondrial superoxide within the Brutal 264.7 tissues are detected utilizing the EPR spin probe mito-TEMPO-H, and therefore adds up within the mitochondia. Inside Profile 2B, the newest mitochondrial nitroxide focus is influenced by subtracting the fresh mito-TEMPO-H rule with time-matched up boundary on the mobile specifications.
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